Human GHRL(Ghrelin) ELISA Kit

SKU: ELK4080

Size:96T

Reactivity:Human

Alternative Names:MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Assay Type:Competitive Inhibition

Sensitivity:22.1 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Endocrinology;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GHRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9UBU6