Human Extracellular glycoprotein lacritin (LACRT) ELISA Kit

SKU: AE36076HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q9GZZ8

Abbreviation: LACRT

Alternative Names: MGC71934;

Application: ELISA

Range: 1.56-100 ng/mL

Sensitivity: 0.63 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate LACRT in samples. An antibody specific for LACRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLACRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LACRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LACRT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Lacritin is a 12.3 kDa glycoprotein encoded in humans by the LACRT gene. Lacritin is a secreted protein found in tears and saliva. Lacritin also promotes tear secretion and proliferation of some epithelial cells. Lacritin is thus a prosecretory mitogen. Most lacritin is produced by the lacrimal gland. Some lacritin is produced by the meibomian gland, and also by epithelial cells of the conjunctiva and cornea. Together these epithelia comprise much of the lacrimal functional unit (LFU). Dry eye is the most common disease of the LFU. Preliminary studies with small trials suggest that lacritin may be differentially downregulated in dry eye. Topical lacritin promotes tearing in rabbit preclinical studies. Lacritin cell targeting is dependent on the cell surface heparan sulfate proteoglycan syndecan-1 (SDC1).

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).