Human ENPP1(Ectonucleotide Pyrophosphatase/Phosphodiesterase 1) ELISA Kit

SKU: ELK3663

Size:96T

Reactivity:Human

Alternative Names:M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1; Membrane component chromosome 6 surface marker 1; Alkaline phosphodiesterase I; Plasma-cell membrane glycoprotein PC-1

Assay Type:Sandwich

Sensitivity:0.064 ng/mL

Standard:10 ng/mL

Detection range:0.16-10 ng/mL

Sample type:serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ENPP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ENPP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ENPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ENPP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P22413