Human Dipeptidase 2 (DPEP2) ELISA Kit

SKU: AE46746HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: Q9H4A9

Abbreviation: DPEP2

Alternative Names: MBD2; OTTHUMP00000174846

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.069 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate DPEP2 in samples. An antibody specific for DPEP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPEP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPEP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPEP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: DPEP2 belongs to the membrane-bound dipeptidase (EC 3.4.13.19) family. These enzymes hydrolyze a variety of dipeptides, including leukotriene D4, the beta-lactam ring of some antibiotics, and cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation.The deduced 578-amino acid mouse protein contains several residues conserved among MBDs, including 6 cysteines, 3 histidines, and a glutamic acid that is important for catalytic activity. Mouse and human MBD2 share 80% amino acid identity. Spleen, liver, and skeletal muscle expressed low Mbd2 levels, and no expression was detected in kidney and brain. Phospholipase treatment released Mbd2 from transfected COS-7 cell membranes, indicating that Mbd2 is anchored to the membrane through glycosylphosphatidylinositol modification.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).