Human Cytochrome P450 3A5, CYP3A5 ELISA Kit

SKU: E3570Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins (PubMed: 2732228, PubMed: 10681376, PubMed: 11093772, PubMed: 12865317). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 12865317, PubMed: 2732228, PubMed: 10681376, PubMed: 11093772). Exhibits high catalytic activity for the formation of catechol estrogens from 17beta-estradiol (E2) and estrone (E1), namely 2-hydroxy E1 and E2 (PubMed: 12865317). Catalyzes 6beta-hydroxylation of the steroid hormones testosterone, progesterone, and androstenedione (PubMed: 2732228). Catalyzes the oxidative conversion of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) (PubMed: 10681376). Further metabolizes all trans-retinoic acid (atRA) to 4-hydroxyretinoate and may play a role in hepatic atRA clearance (PubMed: 11093772). Also involved in the oxidative metabolism of xenobiotics, including calcium Channel blocking drug nifedipine and immunosuppressive drug cyclosporine (PubMed: 2732228).

Research_area:Cardiovascular

Applications:ELISA

Target_protein:CYP3A5

Reactivity:Human

Assay type:Sandwich

Sensitivity:5.14ng/L

Detection range:10-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 3A5

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P20815

MASS(Da):57,109

GeneID:1577

Synonyms:CYP3A5;CYPIIIA5;Cytochrome P450 3A5;Cytochrome P450-PCN3

Gene names:CYP3A5

Standard solution:2400ng/L