Human Cytochrome P450 2C18 (CYP2C18) ELISA Kit

SKU: AE48050HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P33260

Abbreviation: CYP2C18

Alternative Names: RP11-466J14.4; CPCI; CYP2C; CYP2C17; DKFZp686I24235; P450-6B/29C; P450IIC17; (S)-mephenytoin hydroxylase associated cytochrome P450|cytochrome P450 family 2 subfamily C polypeptide 18|cytochrome P45

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Test principle: This assay employs a two-site sandwich ELISA to quantitate CYP2C18 in samples. An antibody specific for CYP2C18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP2C18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2C18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2C18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview:

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).