Human Cytochrome P450 17A1,CYP17A1 ELISA KIT

SKU: E3085Hu

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (PubMed:9452426, PubMed:25301938) (Probable). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:27339894, PubMed:25301938). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938). {ECO:0000269|PubMed:22266943, ECO:0000269|PubMed:25301938, ECO:0000269|PubMed:27339894, ECO:0000269|PubMed:9452426, ECO:0000305|PubMed:8027220}.

Research_area:

Applications:ELISA

Target_protein:CYP17A1

Reactivity:Human

Assay type:Sandwich

Sensitivity:0.09ng/ml

Detection range:0.19-12ng/ml

Sample type:Serum, plasma or other biological fluids

Full product name:Steroid 17-alpha-hydroxylase;17,20 lyase

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P05093

MASS(Da):57371

GeneID:1586

Synonyms:CYP17A1 CYP17 S17AH

Gene names:CYP17A1

Standard solution:24ng/ml