Human Cytochrome Coxidase Subunit 1, MT-CO1 ELISA Kit

SKU: E5154Hu

Size:96 Tests-1 Kit

Background:Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Research_area:Tags & Cell Markers

Applications:ELISA

Target_protein:MT-CO1

Reactivity:Human

Assay type:Sandwich

Sensitivity:7.25ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome c oxidase subunit 1

Species:Human

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:P00395

MASS(Da):57,041

GeneID:4512

Synonyms:COI;COX1;Cytochrome c oxidase polypeptide I;Cytochrome c oxidase subunit 1;MTCO1;MT-CO1

Gene names:MT-CO1

Standard solution:3200ng/L