Human C-C chemokine receptor type 6 (CCR6) ELISA Kit

SKU: AE59130HU

Size: 96T. Other sizes are also available.

Species Reactivity: Human (Homo sapiens)

UniProt: P51684

Abbreviation: CCR6

Alternative Names: BN-1; CD196; CKR-L3; CKR6; CKRL3; CMKBR6; DCR2; DRY-6; GPR-CY4; GPR29; GPRCY4; STRL22; G protein-coupled receptor 29|OTTHUMP00000017618|chemokine (C-C) receptor 6|chemokine receptor-like 3|seven-tra

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.054 ng/mL

Test principle: This assay employs a two-site sandwich ELISA to quantitate CCR6 in samples. An antibody specific for CCR6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCR6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCR6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCR6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Chemokine receptor 6 is a CC chemokine receptor. This protein belongs to family A of G protein-coupled receptor superfamily. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.The gene is located on the long arm of Chromosome 6 (6q27) on the Watson (plus) strand. It is 139,737 bases long and encodes a protein of 374 amino acids.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).