Human Barbiturates ELISA Kit

SKU: ELK8382

Size:96T

Reactivity:Human

Alternative Names:Barbituates

Assay Type:Competitive Inhibition

Sensitivity:0.1 ng/mL

Standard:25 ng/mL

Detection range:0.4-25 ng/mL

Sample type:Plasma, Serum, Urine

Assay length:2h

Research Area:Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: