Human AP1M1(AP-1 Complex Subunit Mu-1) ELISA Kit

SKU: ELK0155

Size:96T

Reactivity:Human

Alternative Names:AP47; CLAPM2; CLTNM; MU-1A; mu1A; Adaptor Related Protein Complex 1 Subunit Mu 1; AP-mu chain family member mu1A

Assay Type:Competitive Inhibition

Sensitivity:0.11 ng/mL

Standard:20 ng/mL

Detection range:0.32-20 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q9BXS5