Human ALOX15(Arachidonate-15-Lipoxygenase) ELISA Kit

SKU: ELK4753

Size:96T

Reactivity:Human

Alternative Names:LOX15; 15-LOX2; 15-LOX1; 15-LOX; 12-LOX; ALOX15; 15-LO; 15-LPG; LOG15; Lipoxygenase 15; Arachidonate 12-lipoxygenase, leukocyte-type; Arachidonate omega-6 lipoxygenase

Assay Type:Sandwich

Sensitivity:0.54 ng/mL

Standard:100 ng/mL

Detection range:1.57-100 ng/mL

Sample type:tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Enzyme & Kinase;Metabolic pathway;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALOX15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALOX15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALOX15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALOX15 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P16050