Horse INS(Insulin) ELISA Kit

SKU: ELK0637

Size:96T

Reactivity:Horse

Alternative Names:Insulin

Assay Type:Sandwich

Sensitivity:68.8 pg/mL

Standard:10000 pg/mL

Detection range:156.25-10000 pg/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Metabolic pathway;Endocrinology;Hormone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse INS in the samples is then determined by comparing the OD of the samples to the standard curve.

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