Golgin 45 rabbit pAb

SKU: ES7816

Size: 100μL

Source:Rabbit

Applications:WB;IHC;IF;ELISA

Reactivity:Human;Mouse

Dilution:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Immunogen:The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:1 mg/ml

Observed_band(KD):47kD

Human_gene_id:8548

Human_swiss_prot_no:Q9H2G9

Subcellular_location:Golgi apparatus membrane .; [Isoform 1]: Nucleus . Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm. .; [Isoform 2]: Cytoplasm . Not detected in the nucleus. .

Other_name:BLZF1; JEM1; Golgin-45; Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor

Background:caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.,