GAPDH Polyclonal Antibody

SKU: E-AB-40337

Size: 100μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Tags/Controls Antibodies

Abbreviation: GAPDH

Target Synonym: aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

Research Areas: Neuroscience;Isotype/Loading Controls;Signal Transduction;Cancer;Metabolism

Conjugation: Unconjugated

Host: Rabbit

Species reactivity: Human;Mouse;Rat

Application: WB;IHC

Isotype: IgG

Clonality: Polyclonal

Clone NO.:

UNIProt ID: Q5XJ10

Accession:

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Concentration: 1 mg/mL

Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

Buffer: PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4

Purification method: Antigen Affinity Purification

Dilution: WB 1:3000-1:6000;IHC 1:300-1:500

Calculated MW: 35 kDa

ObservedMW: 35 kDa