FineTest®488 Anti-Mouse CD22 Antibody(Cy34.1)

SKU: F488-30016

Size: 50T

Target: Mouse CD22

Form: liquid

Conjugation: FineTest®488

Conjugation Information: FineTest® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

Purification: 0

Purity: 0

Host: Mouse

Clonality: Monoclonal

Clone ID: Cy34.1

Isotype: IgG1, κ

Storage: PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Background: The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.

Immunogen: 0

Synonyms: Siglec2|B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22|Cd22|Lyb-8|Sialic acid-binding Ig-like lectin 2|Siglec-2|T-cell surface antigen Leu-14

Observed MW:

Uniprot ID: P35329

Reactivity: Mouse

Tested Application: FC

Recommended dilution: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

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Gene ID: 12483

Research Area:

Catagory: Flow cytometry Antibodies