EasyStep Rat vWF(Von Willebrand Factor) ELISA Kit

SKU: ELK076ES

Size:96T

Reactivity:Rat

Alternative Names:F8VWF; VWD; von Willebrand antigen 2

Assay Type:Sandwich

Sensitivity:41 ng/mL

Standard:10 µg/mL

Detection range:0.16-10 µg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Signal transduction;Hematology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat vWF, and the Rat vWF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat vWF. After TMB substrate solution is added, only those wells that contain Rat vWF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat vWF in the samples is then determined by comparing the OD of the samples to the standard curve.

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