EasyStep Mouse APOB(Apolipoprotein B) ELISA Kit

SKU: ELK094ES

Size:96T

Reactivity:Mouse

Alternative Names:Apo-B; FLDB; Apo B-100; Apo B-48

Assay Type:Sandwich

Sensitivity:43.2 ng/mL

Standard:12 μg/mL

Detection range:0.19-12 μg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOB, and the Mouse APOB standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse APOB. After TMB substrate solution is added, only those wells that contain Mouse APOB and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: