EasyStep Human TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit

SKU: ELK086ES

Size:96T

Reactivity:Human

Alternative Names:EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor

Assay Type:Sandwich

Sensitivity:43.53 pg/mL

Standard:5000 pg/mL

Detection range:78.13-5000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;Hormone metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIMP1, and the Human TIMP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TIMP1. After TMB substrate solution is added, only those wells that contain Human TIMP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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