EasyStep Human Lpa(Lipoprotein a) ELISA Kit

SKU: ELK058ES

Size:96T

Reactivity:Human

Alternative Names:LP-a; LP; AK38; APOA

Assay Type:Sandwich

Sensitivity:0.38 ng/mL

Standard:50 ng/mL

Detection range:0.78-50 ng/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Metabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Lpa, and the Human Lpa standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Lpa. After TMB substrate solution is added, only those wells that contain Human Lpa and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Lpa in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P08519