EasyStep Human GDF15(Growth Differentiation Factor 15) ELISA Kit

SKU: ELK044ES

Size:96T

Reactivity:Human

Alternative Names:PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor

Assay Type:Sandwich

Sensitivity:5.22 pg/mL

Standard:3000 pg/mL

Detection range:46.88-3000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Cytokine;Apoptosis;Tumor immunity;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:Q99988