EasyStep Human Cys-C(CystatinC) ELISA Kit

SKU: ELK005ES

Size:96T

Reactivity:Human

Alternative Names:CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin

Assay Type:Sandwich

Sensitivity:25 pg/mL

Standard:5000 pg/mL

Detection range:78.1-5000 pg/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P01034