EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

SKU: ELK051ES

Size:96T

Reactivity:Human

Alternative Names:25 Hydroxy Vitamin D;

Assay Type:Competitive Inhibition

Sensitivity:7.7 ng/mL

Standard:5000 ng/mL

Detection range:78.13-5000 ng/mL

Sample type:Serum, plasma

Assay length:1.5h

Research Area:Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

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