EasyPure® RNA Kit

SKU: ER101-01

Product Details

Elevate your RNA isolation protocols with the EasyPure® RNA Kit, designed to deliver high-quality RNA from diverse biological samples, including animal cells, animal tissues, bacteria, and yeast. This advanced kit employs a column-based method that ensures rapid and efficient RNA extraction. The process begins with enzymatic lysis of cells and tissues, followed by DNase I treatment to eliminate DNA contamination. RNA is then selectively bound to a silica membrane, washed, and eluted in its pure form, free from protein contaminants.

Features

• Contains DNase I which removes contaminating DNA from RNA samples.
• Safe and Non-Toxic: No need for phenol and chloroform.
• High Yield and Purity: Delivers RNA suitable for RT-PCR, qRT-PCR, and Northern blotting.
• Versatile: Effective for animal cells, tissues, bacteria, and yeast.

See protocol details in manual:

Sample requirements

Material	Amount	Volume of BB4/β-ME
Animal cells	≤5x10^6	0.3-0.6 ml
Animal tissues	≤20 mg	0.3-0.6 ml
Bacterial Cells	≤1x10^9	0.35 ml

*BB4 with β-mercaptoethanol; add 10 μl of β-mercaptoethanol per 1ml of BB4, the solution must be freshly prepared just before use.

Kit Content

Not provided in kit:

• β-mercaptoethanol
• 96%-100% ethanol

Component	ER101-01(50 rxns)
Binding buffer 4 (BB4)	40 ml
Clean buffer 4 (CB4)	60 ml
Wash buffer 4 (WB4)	12 ml
Proteinase K (20 mg/ml)	1 ml
DNase I (3 units/μl)	1500 units
DNase I Reaction Buffer	4×1 ml
RNase-free Water	10 ml
RNase-free Tube (1.5 ml)	50 each
RNA Spin Columns with Collection Tubes	50 each