Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit

SKU: ELK9768

Size:96T

Reactivity:Dog

Alternative Names:Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Assay Type:Competitive Inhibition

Sensitivity:4.07 µg/mL

Standard:1000 µg/mL

Detection range:15.63-1000 µg/mL

Sample type:plasma and erythrocyte lysates

Assay length:2h

Research Area:Diagnosis of diabetes mellitus

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

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