Dog cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

SKU: ELK8127

Size:96T

Reactivity:Dog

Alternative Names:CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac

Assay Type:Sandwich

Sensitivity:24.9 pg/mL

Standard:4000 pg/mL

Detection range:62.5-4000 pg/mL

Sample type:Serum, plasma, tissue homogenates and other biological fluids

Assay length:3.5h

Research Area:Cardiovascular biology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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