DMT Chemically Competent Cell

SKU: CD511-01

• DMT Chemical Competent cells have high transformation efficiency: >10⁸ cfu/μg (pUC19 DNA), are resistant to T1 and T5 phage. The cells harbor DMT enzyme (DpnI) to achieve in vivo digestion of methylated DN and are ideal for some site-directed mutagenesis protocols.

Genotype	F- φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 gyrA96relA1 tonA
Features	• High transformation efficiency: >108 cfu/μg (pUC19 DNA). • Resistance to T1 and T5 phage. • In vivo digestion of methylated DNA, suitable for site-directed mutagenesis.
Storage	at -70 °C for six months
Notes	Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure.

References:

Literature	Journal	IF	Author	Date	Link
Characterization of EndoTT, a novel single-stranded DNA-specific endonuclease from Thermoanaerobacter tengcongensis	nucleic acids research	9.202	Zhang S, et al. State Key Laboratory of Microbial	2010 Jun	The original link
Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis	Biotechnology for Biofuels	6.4439	Nanyu Han,et al.	2017 May	The original link
A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity	Front Cell Infect Microbiol	5.2179	Bingqing Li,et al.	2017Feb	The original link
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/??-Glucanase That Can Degrade Crystalline Cellulose	Applied and Environmental Microbiology	3.668	Xianli Xue, et al.	2015 Mar	The original link