CRHR1 ELISA kit (Rat)

SKU: SEC935Ra

Size: 96Tests

# of Times Cited in literature: -

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: CRHR1

Target Full Name: Corticotropin Releasing Hormone Receptor 1

Alternative Names: CRF-R; CRF1; CRFR1; CRH-R1h; CRHR; CRHR1f; Corticotropin-releasing factor receptor 1

Target Species: Rat

Uniprot: P35353

Gene ID: 58959

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat CRHR1 / Corticotropin Releasing Hormone Receptor 1

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.312-20ng/mL

Sensitivity: 0.128ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 1 (CRHR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Hormone Receptor 1 (CRHR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone Receptor 1 (CRHR1). No significant cross-reactivity or interference between Corticotropin Releasing Hormone Receptor 1 (CRHR1) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 5 times; 5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 6. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Corticotropin Releasing Hormone Receptor 1 (CRHR1) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Releasing Hormone Receptor 1 (CRHR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Endocrinology;

References Citing This Product: Involvement of CRH Receptors in the Neuroprotective Action of R-Apomorphine in the Striatal 6-OHDA Rat Model

Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome