CPP ELISA kit (Human)

SKU: SEA365Hu

Size: 96Tests

# of Times Cited in literature: 20

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: CPP

Target Full Name: Copeptin

Alternative Names: C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin

Target Species: Human

Uniprot: P01185

Gene ID: 551

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human CPP / Copeptin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 15.6-1,000pg/mL

Sensitivity: 5.9pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Copeptin (CPP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Copeptin (CPP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Copeptin (CPP). No significant cross-reactivity or interference between Copeptin (CPP) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Copeptin (CPP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Copeptin (CPP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Copeptin (CPP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Copeptin (CPP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: -

References Citing This Product: Serum copeptin in children exposed to maltreatment

Orthostatic Challenge Shifts the Hemostatic System of Patients Recovered from Stroke toward Hypercoagulability.

Evaluation of Urinary Aquaporin 2 and Plasma Copeptin as Biomarkers of Effectiveness of Desmopressin Acetate for the Treatment of Monosymptomatic Nocturnal Enuresis.

Copeptin is independently associated with vascular calcification in chronic kidney disease stage 5

Micro RNA 146a gene variant/TNF-α/IL-6/IL-1 β A cross-link axis inbetween oxidative stress, endothelial dysfunction and neuro-inflammation in acute ischemic stroke …

Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study

Relation of Copeptin with Diabetic and Renal Function Markers Among Patients with Diabetes Mellitus Progressing Towards Diabetic Nephropathy

重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响

Validity of different copeptin assays in the differential diagnosis of the polyuria-polydipsia syndrome

Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women?

Copeptin levels are more elevated and associated with cardiovascular events in the unity of chronic ischemic heart and chronic kidney diseases

The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose