CHEM ELISA kit (Rat)

SKU: SEA945Ra

Size: 96Tests

# of Times Cited in literature: 8

Prepare Time: 2-7 days(please inquire for mutiple units)

Target Name: CHEM

Target Full Name: Chemerin

Alternative Names: TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

Target Species: Rat

Uniprot: Q5BK77

Gene ID: 297073

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Rat CHEM / Chemerin

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 15.6-1,000pg/mL

Sensitivity: 6.9pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM). No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemerin (CHEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemerin (CHEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemerin (CHEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemerin (CHEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Cytokine;Metabolic pathway;Infection immunity;

References Citing This Product: Expression and regulation of chemerin during rat pregnancy

Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats

Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.

Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats

Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats

Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes

Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …

The decline of whole-body glucose metabolism in ovariectomized rats

Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin

Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model