Cattle IL12(Interleukin 12) ELISA Kit

SKU: ELK10926

Size:96T

Reactivity:Cattle

Alternative Names:IL-12;Interleukin 12; IL12/P70; Interleukin-12/P70

Assay Type:Sandwich

Sensitivity:6.1 pg/mL

Standard:1000 pg/mL

Detection range:15.63-1000 pg/mL

Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length:3.5h

Research Area:Cytokine;Infection immunity;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL12 in the samples is then determined by comparing the OD of the samples to the standard curve.

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