Cattle HB(Hemoglobin) ELISA Kit

SKU: ELK8165

Size:96T

Reactivity:Cattle

Alternative Names:Hgb; Haemoglobin; Heterotetramer(αβ)2

Assay Type:Competitive Inhibition

Sensitivity:5.7 µg/mL

Standard:1000 µg/mL

Detection range:15.63-1000 µg/mL

Sample type:Serum, plasma, erythrocyte lysates and other biological fluids

Assay length:2h

Research Area:Metabolic pathway;Infection immunity;Hematology;Neuro science;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle HB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HB in the samples is then determined by comparing the OD of the samples to the standard curve.

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