Cattle CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

SKU: ELK9956

Size:96T

Reactivity:Cattle

Alternative Names:CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule

Assay Type:Sandwich

Sensitivity:0.221 ng/mL

Standard:40 ng/mL

Detection range:0.63-40 ng/mL

Sample type:serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay length:3.5h

Research Area:Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID:P51749