Cat IgA(Immunoglobulin A) ELISA Kit

SKU: ELK11423

Size:96T

Reactivity:Cat

Alternative Names:Immunoglobulin A

Assay Type:Sandwich

Sensitivity:0.07 ug/mL

Standard:100 ug/mL

Detection range:1.57-100 ug/mL

Sample type:serum, plasma, saliva and other biological fluids

Assay length:3.5h

Research Area:Infection immunity;Immune molecule;Hematology;Pulmonology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cat IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cat IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cat IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cat IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

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