GeneBio Systems

CASP3 ELISA kit (Mouse)

Name: CASP3 ELISA kit (Mouse)
Brand: GeneBio Systems
SKU: SEA626Mu
Price: 170400 JPY
Availability: InStock

SKU:SEA626Mu

¥170,400 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 18

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: CASP3

Target Full Name: Caspase 3

Alternative Names: CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1

Target Species: Mouse

Uniprot: P70677

Gene ID: 12367

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Mouse CASP3 / Caspase 3

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.625-40ng/mL

Sensitivity: 0.229ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 3 (CASP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3). No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 3 (CASP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 3 (CASP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 3 (CASP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 3 (CASP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity;

References Citing This Product: Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells.

Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats

Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis

Inhibition of small HA fragment activity and stimulation of A2A

The heat shock protein 90 inhibitor, 17-AAG, attenuates thioacetamide induced liver fibrosis in mice

An integrated proteomics approach shows synaptic plasticity changes in an APP/PS1 Alzheimers mouse model

Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice

Chronic low-dose-rate ionising radiation affects the hippocampal phosphoproteome in the ApoE−/− alzheimer mouse model

Protective effects of apigenin and myricetin against cisplatin-induced nephrotoxicity in mice.

Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice.

Tectorigenin attenuates the MPP+ induced SH‑SY5Y cell damage, indicating a potential beneficial role in Parkinson's disease by oxidative stress inhibition