C3 ELISA kit (Bovine)

SKU: SEA861Bo

Size: 96Tests

# of Times Cited in literature: 5

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: C3

Target Full Name: Complement Component 3

Alternative Names: ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

Target Species: Bovine

Uniprot: Q2UVX4

Gene ID: 280677

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Bovine C3 / Complement Component 3

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 3.12-200ng/mL

Sensitivity: 1.28ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3 (C3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3 (C3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 3 (C3). No significant cross-reactivity or interference between Complement Component 3 (C3) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complement Component 3 (C3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complement Component 3 (C3) and unlabeled Complement Component 3 (C3) (Standards or samples) with the pre-coated antibody specific to Complement Component 3 (C3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complement Component 3 (C3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complement Component 3 (C3) in the sample.

Research Area: Signal transduction;Infection immunity;Immune molecule;

References Citing This Product: Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin

Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk

The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain.

The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte

Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves