C1QTNF3 ELISA kit (Human)

SKU: SEK169Hu

Size: 96Tests

# of Times Cited in literature: 7

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: C1QTNF3

Target Full Name: C1q And Tumor Necrosis Factor Related Protein 3

Alternative Names: CORS26; CTRP3; Corcs; Cors; Cartonectin; Collagenous repeat-containing sequence 26 kDa protein

Target Species: Human

Uniprot: Q9BXJ4

Gene ID: 114899

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human C1QTNF3 / C1q And Tumor Necrosis Factor Related Protein 3

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.055ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3). No significant cross-reactivity or interference between C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Tumor immunity;

References Citing This Product: Low Serum Cartonectin/CTRP3 Concentrations in Newly Diagnosed Type 2 Diabetes Mellitus: In Vivo Regulation of Cartonectin by Glucose

Serum levels of C1q/TNF-related protein-1 (CTRP-1) are closely associated with coronary artery disease

Plasma C1q/TNF-Related Protein-3 (CTRP-3) and High-Mobility Group Box-1 (HMGB-1) Concentrations in Subjects with Prediabetes and Type 2 Diabetes

Circulating C1q/TNF-Related Protein 3, Omentin-1 and NGAL in Obese Patients with Type 2 Diabetes During Insulin Therapy