ASD ELISA kit (General species)

SKU: CEA456Ge

Size: 96Tests

# of Times Cited in literature: 8

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ASD

Target Full Name: Androstenedione

Alternative Names: 4-Androstenedione; 4-Androstene-3,17-Dione

Target Species: General species

Uniprot: -

Gene ID: -

Featured Series: CE kit

Featured Series Function: Detects small molecule

Specificity: Reactive with General species ASD / Androstenedione

Method: Colormetric

Detection principle: Competitive Inhibition

Detection range: 123.5-10,000pg/mL

Sensitivity: 44.2pg/mL

Assay Time: 2h

Sample Size: 50uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Androstenedione (ASD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Androstenedione (ASD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Androstenedione (ASD). No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androstenedione (ASD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androstenedione (ASD) and unlabeled Androstenedione (ASD) (Standards or samples) with the pre-coated antibody specific to Androstenedione (ASD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androstenedione (ASD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androstenedione (ASD) in the sample.

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

References Citing This Product: Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota

Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus

Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.

Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome

Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells

Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries

Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells

A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats

Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis