GeneBio Systems

ANXA2 ELISA kit (Human)

Name: ANXA2 ELISA kit (Human)
Brand: GeneBio Systems
SKU: SEB944Hu
Price: 174900 JPY
Availability: InStock

SKU:SEB944Hu

¥174,900 JPY

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Quantity

Size: 96Tests

# of Times Cited in literature: 19

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: ANXA2

Target Full Name: Annexin A2

Alternative Names: ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV

Target Species: Human

Uniprot: P07355

Gene ID: 302

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human ANXA2 / Annexin A2

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.054ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2). No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A2 (ANXA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A2 (ANXA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A2 (ANXA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A2 (ANXA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Signal transduction;Tumor immunity;Bone metabolism;

References Citing This Product: Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis

Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma

Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis

Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma

Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis

Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables

Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B

Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma

Circulating

Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma

Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population