Anti-ZW10 Antibody Picoband®

SKU: A04266-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ZW10

Specificity:

Background: Centromere/kinetochore protein zw10 homolog is a protein that in humans is encoded by the ZW10 gene This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis.

Immunogen: E.coli-derived human ZW10 recombinant protein (Position: K35-K779).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chan, G. K. T., Jablonski, S. A., Starr, D. A., Goldberg, M. L., Yen, T. J. Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores. Nature Cell Biol. 2: 944-947, 2000. 2. Musio, A., Mariani, T., Montagna, C., Zambroni, D., Ascoli, C., Ried, T., Vezzoni, P. Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. Gene 331: 33-40, 2004. 3. Scaerou, F., Starr, D. A., Piano, F., Papoulas, O., Karess, R. E., Goldberg, M. L. The ZW10 and rough deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore. J. Cell Sci. 114: 3103-3114, 2001.

Uniprot ID: O43264

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 95 kDa

Calculated Molecular Weight:

Gene ID: 9183

Protein Name: Centromere/kinetochore protein zw10 homolog

Gene Full Name: zw10 kinetochore protein

Synonyms: HZW10; KNTC1AP; ZW10