Anti-YY1 Antibody Picoband® (monoclonal, 3F3E7)

SKU: M00833-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: YY1

Specificity:

Background: YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human YY1, identical to the related mouse sequence.

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Shi Y, Seto E, Chang LS, Shenk T (Oct 1991). Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein. Cell 67 (2): 377–88. 2. Thiaville MM, Kim J (2011). Oncogenic potential of yin yang 1 mediated through control of imprinted genes. Critical Reviews in Oncogenesis 16 (3-4): 199–209. 3. Zhu W, Lossie AC, Camper SA, Gumucio DL (Apr 1994). Chromosomal localization of the transcription factor YY1 in the mouse and human. Mammalian Genome 5 (4): 234–6.

Uniprot ID: P25490

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 3F3E7

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight:

Gene ID: 7528

Protein Name: Transcriptional repressor protein YY1

Gene Full Name: YY1 transcription factor

Synonyms: DELTA; Delta transcription factor; INO80 complex subunit S; INO80S; NF E1; UCRBP; Yin and yang 1; YIN YANG 1; YY 1; YY1; YY1 transcription factor