Anti-VPS36 Antibody Picoband®

SKU: A07809-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml/ml, Human

Gene Name: VPS36

Specificity:

Background: Vacuolar protein-sorting-associated protein 36 is a protein that in humans is encoded by the VPS36 gene. This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II.

Immunogen: E.coli-derived human VPS36 recombinant protein (Position: M1-S386).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Alam, S. L., Langelier, C., Whitby, F. G., Koirala, S., Robinson, H., Hill, C. P., Sundquist, W. I. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nature Struct. Biol. 13: 1029-1030, 2006. 2. Kamura, T., Burian, D., Khalili, H., Schmidt, S. L., Sato, S., Liu, W.-J., Conrad, M. N., Conaway, R. C., Conaway, J. W., Shilatifard, A. Cloning and characterization of ELL-associated proteins EAP45 and EAP20: a role for yeast EAP-like proteins in regulation of gene expression by glucose. J. Biol. Chem. 276: 16528-16533, 2001. 3. Slagsvold, T., Aasland, R., Hirano, S., Bache, K. G., Raiborg, C., Trambaiolo, D., Wakatsuki, S., Stenmark, H. Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain. J. Biol. Chem. 280: 19600-19606, 2005.

Uniprot ID: Q86VN1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 44 kDa

Calculated Molecular Weight: 49229 MW

Gene ID: 51028

Protein Name: Vacuolar protein-sorting-associated protein 36

Gene Full Name: vacuolar protein sorting 36 homolog

Synonyms: C13orf9; CGI 145; EAP45; ESCRT II complex subunit VPS36; VPS36