Anti-Vinculin/VCL Antibody Picoband®

SKU: PA1781

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Gene Name: VCL

Specificity: No cross reactivity with other proteins.

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ezzell RM, Goldmann WH, Wang N, Parashurama N, Parasharama N, Ingber DE (1997). Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton. Exp. Cell Res. 231 (1): 14–26. 2. Goldmann WH, Ingber DE (2001). Intact vinculin protein is Require for control of cell shape, cell mechanics, and rac-Dependent Lamellipodia Formation. Biochemical and Biophysical Research Communications. 290 (2): 749–755. 3. Xu W, Baribault H, Adamson ED (1998). vinculin knockout results in heart and brain defects during embryonic development. Development. 125(2): 327–337.

Uniprot ID: P18206

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 34 kDa

Calculated Molecular Weight: 123799 MW

Gene ID: 7414

Protein Name: Vinculin

Gene Full Name: vinculin

Synonyms: CMD1W; Metavinculin; MVCL; VCL; Vinculin