Anti-USP14 Antibody Picoband®

SKU: A03042-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: USP14

Specificity:

Background: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen: E.coli-derived human USP14 recombinant protein (Position: D23-Q427). Human USP14 shares 96.3% amino acid (aa) sequence identity with mouse USP14.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Dennehey, B. K., Gutches, D. G., McConkey, E. H., Krauter, K. S. Inversion, duplication, and changes in gene context are associated with human chromosome 18 evolution. Genomics 83: 493-501, 2004. 2. Deshpande, K. L., Seubert, P. H., Tillman, D. M., Farkas, W. R., Katze, J. R. Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit. Arch. Biochem. Biophys. 326: 1-7, 1996. 3. Gross, M. B. Personal Communication. Baltimore, Md. 7/13/2015.

Uniprot ID: P54578

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 9097

Protein Name: Ubiquitin carboxyl-terminal hydrolase 14

Gene Full Name: ubiquitin specific peptidase 14

Synonyms: Deubiquitinating enzyme 14; TGT; Ubiquitin thiolesterase 14; USP14