Anti-UNG Antibody Picoband®

SKU: A01672-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: UNG

Specificity:

Background: Uracil-DNA glycosylase, also known as UNG or UDG, is a human gene though orthologs exist ubiquitously among prokaryotes and eukaryotes and even in some DNA viruses. The first uracil DNA-glycosylase was isolated from Escherichia coli. This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene, which has been confused with this gene, in the literature and some databases.

Immunogen: E.coli-derived human UNG recombinant protein (Position: E96-L313).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Lindahl T, Ljungquist S, Siegert W, Nyberg B, Sperens B (May 1977). DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli. The Journal of Biological Chemistry. 252 (10): 3286–94. 2. Longo MC, Berninger MS, Hartley JL (Sep 1990). Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene. 93 (1): 125–8. 3. Pearl LH (Aug 2000). Structure and function in the uracil-DNA glycosylase superfamily. Mutation Research. 460 (3-4): 165–81.

Uniprot ID: P13051

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 50514 MW

Gene ID: 7374

Protein Name: Uracil-DNA glycosylase

Gene Full Name: uracil DNA glycosylase

Synonyms: DGU; HIGM4; UDG; UNG; UNG1; UNG15; UNG2; uracil DNA glycosylase