Anti-UBE2D1/2/3/4 Antibody Picoband®

SKU: A04728-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -

Gene Name: UBE2D1/2/3/4

Specificity:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human UBE2D1/2/3/4 recombinant protein (Position: D116-M147).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Gehrke, S. G., Riedel, H.-D., Herrmann, T., Hadaschik, B., Bents, K., Veltkamp, C., Stremmel, W. UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood 101: 3288-3293, 2003. 2. Gutierrez, J. A., Yu, J., Rivera, S., Wessling-Resnick, M. Functional expression cloning and characterization of SFT, a stimulator of Fe transport. J. Cell Biol. 139: 895-905, 1997. Note: Erratum: J. Cell Biol. 147: 205 only, 1999. 3. Gutierrez, J. A., Yu, J., Wessling-Resnick, M. Characterization and chromosomal mapping of the human gene for SFT, a stimulator of Fe transport. Biochem. Biophys. Res. Commun. 253: 739-742, 1998.

Uniprot ID: P51668/P62837/P61077/Q9Y2X8

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17 kDa

Calculated Molecular Weight: 32193 MW

Gene ID: 7321/7322/7323/51619

Protein Name: Ly6-A antigen

Gene Full Name: ubiquitin conjugating enzyme E2 D1/2/3/4

Synonyms: E2(17)KB1; SFT; Stimulator of Fe transport; UBC4/5; UBC4/5 homolog; UBC5A; UBCH5; UBCH5A; UBE2D; UBE2D1; UBE2D1/2/3/4; UBE2D2; UBE2D3; UBE2D4; Ubiquitin carrier protein D1; Ubiquitin protein ligase D1; E2(17)KB2; PUBC1; UBC4; UBC5B; UBCH4; UBCH5B; Ubiquitin carrier protein D2; Ubiquitin protein ligase D2; E2(17)KB3; UBC5C; UBCH5C; Ubiquitin carrier protein D3; Ubiquitin protein ligase D3; HBUCE1; UBCH5D; Ubiquitin carrier protein D4; Ubiquitin protein ligase D4