Anti-TSH Receptor/TSHR Antibody Picoband®

SKU: A00576-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Gene Name: TSHR

Specificity: No cross reactivity with other proteins.

Background: TSHR (Thyroid-Stimulating Hormone Receptor), also called LGR3, is mapped to 14q31.1. The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human TSH Receptor, different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Akamizu, T., Ikuyama, S., Saji, M., Kosugi, S., Kozak, C., McBride, O. W., Kohn, L. D. Cloning, chromosomal assignment, and regulation of the rat thyrotropin receptor: expression of the gene is regulated by thyrotropin, agents that increase cAMP levels, and thyroid autoantibodies. Proc. Nat. Acad. Sci. 87: 5677-5681, 1990. 2. Dechairo, B. M., Zabaneh, D., Collins, J., Brand, O., Dawson, G. J., Green, A. P., Mackay, I., Franklyn, J. A., Connell, J. M., Wass, J. A. H., Wiersinga, W. M., Hegedus, L., Brix, T., Robinson, B. G., Hunt, P. J., Weetman, A. P., Carey, A. H., Gough, S. C. Association of the TSHR gene with Graves' disease: the first disease-specific locus. Europ. J. Hum. Genet. 13: 1223-1230, 2005. 3. Wadsworth, H. L., Chazenbalk, G. D., Nagayama, Y., Russo, D., Rapoport, B. An insertion in the human thyrotropin receptor critical for high affinity hormone binding. Science 249: 1423-1425, 1990.

Uniprot ID: P16473

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40, 70-80, 110 kDa

Calculated Molecular Weight: 87 kDa

Gene ID: 7253

Protein Name: Thyrotropin receptor

Gene Full Name: thyroid stimulating hormone receptor

Synonyms: CHNG1; hTSHR I; LGR3; Thyrotropin receptor; TSH R; TSHR