Anti-TRMT6 Antibody Picoband®

SKU: A13158

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TRMT6

Specificity:

Background: This gene encodes a member of the tRNA methyltransferase 6 protein family. A similar protein in yeast is part of a two component methyltransferase, which is involved in the posttranslational modification that produces the modified nucleoside 1-methyladenosine in tRNAs. Modified 1-methyladenosine influences initiator methionine stability and may be involved in the replication of human immunodeficiency virus type 1. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human TRMT6 recombinant protein (Position: E6-K489).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Wang, Y. , Huang, Q. , Deng, T. , Li, B. H. , & Ren, X. Q. . (2019). Clinical significance of trmt6 in hepatocellular carcinoma: a bioinformatics-based study. Medical Science Monitor, 25, 3894-3901. 2. Finermoore, J. , Czudnochowski, N. , O'Connell, J. D. , Wang, A. L. , & Stroud, R. M. . (2015). Crystal structure of the human trna m1a58 methyltransferase–trna3lys complex: refolding of substrate trna allows access to the methylation target. Journal of Molecular Biology, 427(24), 3862-3876. 3. Wang, Y. , Wang, J. , Li, X. , Xiong, X. , Wang, J. , & Zhou, Z. , et al. N 1-methyladenosine methylation in trna drives liver tumourigenesis by regulating cholesterol metabolism. Nature communications, 12(1), 6314.

Uniprot ID: Q9UJA5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 9700 MW

Gene ID: 51605

Protein Name: tRNA

Gene Full Name: tRNA methyltransferase 6 non-catalytic subunit

Synonyms: CGI 09; GCD10; Gcd10p; KIAA1153; TRM6; TRMT6; tRNA(m1A58)MTase subunit TRM6