Anti-TCEB2/Elongin-B/ELOB Antibody Picoband®

SKU: A31718-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ELOB

Specificity:

Background: Elongin B is a protein that in humans is encoded by the ELOB gene. This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Immunogen: E.coli-derived human TCEB2/Elongin-B/ELOB recombinant protein (Position: M1-Q118).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aso, T., Lane, W. S., Conaway, J. W., Conaway, R. C. Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II. Science 269: 1439-1443, 1995. 2. Duan, D. R., Pause, A., Burgess, W. H., Aso, T., Chen, D. Y. T., Garrett, K. P., Conaway, R. C., Conaway, J. W., Linehan, W. M., Klausner, R. D. Inhibition of transcription elongation by the VHL tumor suppressor protein. Science 269: 1402-1406, 1995. 3. Kibel, A., Iliopoulos, O., DeCaprio, J. A., Kaelin, W. G., Jr. Binding of the von Hippel-Lindau tumor suppressor protein to elongin B and C. Science 269: 1444-1446, 1995.

Uniprot ID: Q15370

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 6923

Protein Name: Elongin-B

Gene Full Name: elongin B

Synonyms: ELOB; Elongin 18 kDa subunit; Elongin B; SIII; SIII p18; TCEB2