Anti-SLD5/GINS4 Antibody Picoband®

SKU: A10548-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GINS4

Specificity:

Background: The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.

Immunogen: E.coli-derived human SLD5/GINS4 recombinant protein (Position: M1-I223). Human GINS4 shares 87.9% and 88.8% amino acid (aa) sequence identity with mouse and rat GINS4, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kong, L., Ueno, M., Itoh, M., Yoshioka, K., Takakura, N. Identification and characterization of mouse PSF1-binding protein, SLD5. Biochem. Biophys. Res. Commun. 339: 1204-1207, 2006. 2. Takayama, Y., Kamimura, Y., Okawa, M., Muramatsu, S., Sugino, A., Araki, H. GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev. 17: 1153-1165, 2003. 3. Ueno, M., Itoh, M., Kong, L., Sugihara, K., Asano, M., Takakura, N. PSF1 is essential for early embryogenesis in mice. Molec. Cell Biol. 25: 10528-10532, 2005.

Uniprot ID: Q9BRT9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 26 kDa

Gene ID: 84296

Protein Name: DNA replication complex GINS protein SLD5

Gene Full Name: GINS complex subunit 4

Synonyms: GINS4; SLD5; DNA replication complex GINS protein SLD5; GINS complex subunit 4 [Cleaved into: DNA replication complex GINS protein SLD5, N-terminally processed]